1st Reverse Motion in Film

Demolition of a Wall (1896) - Louis Lumiere

In Demolition of a Wall (aka Démolition d'un mur) by Louis Lumière, we see the action proceeding forward as expected, but at the mid-point of the film, the footage is reversed, taking us back to the beginning. This was the 1st movie to contain footage in reverse motion.

http://www.imdb.com/title/tt0000070/